Microalgae enhanced co-metabolism of sulfamethoxazole using aquacultural feedstuff components: Co-metabolic pathways and enzymatic mechanisms.

The application of antibiotics in freshwater aquaculture leads to increased contamination of aquatic environments. However, limited information is available on the co-metabolic biodegradation of antibiotics by microalgae in aquaculture. Feedstuffs provide multiple organic substrates for microalgae-mediated co-metabolism. Herein, we investigated the co-metabolism of sulfamethoxazole (SMX) by Chlorella pyrenoidosa when adding main components of feedstuff (glucose and lysine). Results showed that lysine had an approximately 1.5-fold stronger enhancement on microalgae-mediated co-metabolism of SMX than glucose, with the highest removal rate (68.77% ± 0.50%) observed in the 9-mM-Lys co-metabolic system. Furthermore, we incorporated reactive sites predicted by density functional theory calculations, 14 co-metabolites identified by mass spectrometry, and the roles of 18 significantly activated enzymes to reveal the catalytic reaction mechanisms underlying the microalgae-mediated co-metabolism of SMX. In lysine- and glucose-treated groups, five similar co-metabolic pathways were proposed, including bond breaking on the nucleophilic sulfur atom, ring cleavage and hydroxylation at multiple free radical reaction sites, together with acylation and glutamyl conjugation on electrophilic nitrogen atoms. Cytochrome P450, serine hydrolase, and peroxidase play crucial roles in catalyzing hydroxylation, bond breaking, and ring cleavage of SMX. These findings provide theoretical support for better utilization of microalgae-driven co-metabolism to reduce sulfonamide antibiotic residues in aquaculture.

In situ single iron atom doping on Bi2WO6 monolayers triggers efficient photo-fenton reaction.

Developing an efficient photocatalytic system for hydrogen peroxide (H2O2) activation in Fenton-like processes holds significant promise for advancing water purification technologies. However, challenges such as high carrier recombination rates, limited active sites, and suboptimal H2O2 activation efficiency impede optimal performance. Here we show that single-iron-atom dispersed Bi2WO6 monolayers (SIAD-BWOM), designed through a facile hydrothermal approach, can offer abundant active sites for H2O2 activation. The SIAD-BWOM catalyst demonstrates superior photo-Fenton degradation capabilities, particularly for the persistent pesticide dinotefuran (DNF), showcasing its potential in addressing recalcitrant organic pollutants. We reveal that the incorporation of iron atoms in place of tungsten within the electron-rich [WO4]2- layers significantly facilitates electron transfer processes and boosts the Fe(II)/Fe(III) cycle efficiency. Complementary experimental investigations and theoretical analyses further elucidate how the atomically dispersed iron induces lattice strain in the Bi2WO6 monolayer, thereby modulating the d-band center of iron to improve H2O2 adsorption and activation. Our research provides a practical framework for developing advanced photo-Fenton catalysts, which can be used to treat emerging and refractory organic pollutants more effectively.

Comparative Analysis of Clinical Outcomes: Posterior Cervical Endoscopic Discectomy versus Fenestration Laminectomy Discectomy for Cervical Disc Herniation.

Objective: It aimed to investigate the difference in clinical efficacy between posterior cervical endoscopic discectomy (PCED) and Fenestration laminectomy discectomy (FLD) in cervical disc herniation (CDH). Methods: This retrospective study analyzed 100 CDH patients undergoing nucleotomy and assigned them into the FLD and PCED groups, 50 cases for each group. The differences in operation time, intraoperative blood loss, skin incision, off-bed time, and hospital stay were evaluated. Numeric rating scales (NRS), Oswestry disability Index (ODI), Japanese Orthopaedic Association (JOA), excellent and good clinical efficacy, quality of life (QoL) SF-36 score, and complication rate were compared. Results: The results showed that compared with the FLD group, the PCED group had increased operation time, decreased intraoperative blood loss, skin incision length, off-bed time, and hospital stay (P < .01). Compared with the FLD group, the PCED group had decreased NRS and ODI scores and increased JOA scores at 1 d, 3 d, 1 month, 3 months, 6 months, 12 months, and 24 months after operation (P < .05). Compared with the FLD group, the excellent and good rate of the PCED group increased significantly after 6 months, 1 year, and 2 years (52.0% vs 64.0%, 58.0% vs. 80.0%, 68.0% vs 90.0%, P < .05). Relative to the FLD group, the physical function, emotional function, vitality, social function, and mental health score of the PCED group increased obviously at 2 years after operation (P < .01). The postoperative complication rate was 0% in both FLD and PCED groups. PCED has good long-term clinical efficacy in the treatment of CDH, with excellent recovery and high safety. Conclusion: PCED showed favorable long-term clinical efficacy in the treatment of CDH, with excellent recovery and high safety. Compared to FLD, PCED resulted in reduced intraoperative blood loss, shorter incision length, and faster recovery. It also led to improved pain scores, functional outcomes, and quality of life measures. The absence of postoperative complications further supports the use of PCED as an effective treatment option for CDH.

Influence of crop residue-induced Fe-DOC complexation on nitrate reduction in paddy soil.

Although complexation between dissolved organic matter (DOM) and ubiquitous Fe is known to have a major influence on electron transferring ability in redoximorphic soil, it was unclear whether and how this complexation affected nitrate reduction and N2O productivity. The nitrate reduction of paddy soil in the presence of crop residues returning under flooding conditions was explored in this study. The rate of nitrate reduction in control soil was 0.0677 d-1, while it improved 1.99 times in treatment soil with Chinese milk vetch (CMV) straw returning. During a 28-day incubation period, N2O productivity decreased 0.08-0.91 ppb in CMV soil and 0.43-0.50 ppb in rice straw soil compared with control. The presence of crop residue increased DOC content and Fe (III) reduction rate, which aided in the formation of Fe (II)-DOC complexation. Meanwhile, the addition of CMV increased the content of DOC by 5.14-78.77 mg/kg and HCl extractable Fe (II) by 35.12-1221.03 mg/kg. Crop residues returning to soil increased the relative abundance of iron reductive and electroactive genera, as well as denitrifying genera with more copies of denitrification genes (Archangiaceae, Gemmatimonadaceae, and Burkholderiaceae). The synergistic effect of Fe-DOC complexation, electroactive genera, and denitrifying genera contributed to up-regulated expression of napA and narG (5.84 × 106 and 3.39 × 107 copies increased in the CMV soil compared to the control) numbers and equally accelerated reduction of nitrate to nitrite, while further nitrite reduction was primarily attributed to the abiotic reaction by Fe (II). From a bio-electrochemical point of view, this work provided new insight into the nitrate reduction of paddy soil impacted by Fe-DOC complexation.

Mass variations and transfer process of shrimp farming pollutants in aquaculture drainage systems: Effects of DOM features and physicochemical properties.

The expansion of aquaculture produces increasing pollutant loads, necessitating the use of drainage systems to discharge wastewater into surface water. To assess the mass variations and transfer process of aquaculture wastewater, an entire aquaculture drainage investigation lasting for 48 h was conducted, focusing on the nutrients, heavy metals, dissolved organic matter (DOM), and physicochemical properties of drainage in a commercial shrimp farm. The findings revealed that early drainage produced more heavy metals, total nitrogen (TN), dissolved organic nitrogen (DON), and feed-like proteins from aquaculture floating feed and additives, whereas late drainage produced more PO43--P and total dissolved phosphorus (TP). A few pollutants, including DON, Cu, and feed-like proteins, were effectively removed, whereas the contents of TN, dissolved inorganic nitrogen, and Zn increased in the multi-level aquaculture drainage system. Limited dilution indicated that in-stream transfer was the main process shaping pollutant concentrations within the drainage system. In the lower ditches, NO3--N, heavy metals, and feed-like proteins exhibited evident in-stream attenuation, while TN and NH4+-N underwent significant in-stream enrichment processes, especially in ditch C, with the transfer coefficient values (vf) of -1.74E-5 and -2.04E-5. This indicates that traditional aquaculture drainage systems serve as nitrogen sinks, rather than efficient nutrient purge facilitators. Notably, DOM was identified as a more influential factor in shaping the in-stream transfer process in aquaculture drainage systems, with an interpretation rate 40.79% higher than that of the physiochemical properties. Consequently, it is necessary to eliminate the obstacles posed by DOM to pollutant absorption and net zero emissions in aquaculture drainage systems in the future. ENVIRONMENTAL IMPLICATIONS: Nutrients, heavy metals, and dissolved organic matter are hazardous pollutants originating from high-density aquaculture. As the sole conduit to natural waters, aquaculture drainage systems have pivotal functions in receiving and purifying wastewater, in which the in-stream transfer process is affected by ambient conditions. This field study investigated the spatial variations, stage distinctions, effects of physicochemical properties, and dissolved organic matter (DOM) features. This finding suggests that the aquaculture drainage system as a nitrogen sink and DOM source. While the DOM is the key factor in shaping the in-stream transfer process, and obstacles for pollutant elimination. This study helps in understanding the fate of aquaculture pollutants and reveals the drawbacks of traditional aquaculture drainage systems.

Nitrate input inhibited the biodegradation of erythromycin through affecting bacterial network modules and keystone species in lake sediments.

Antibiotic contamination and excessive nitrate loads are generally concurrent in aquatic ecosystems. However, little is known about the effects of nitrate input on the biodegradation of antibiotics. In this study, the effects of nitrate input on microbial degradation of erythromycin, a typical macrolide antibiotic widely detected in lake sediments, were investigated. The results showed that the nitrate input significantly inhibited the erythromycin removal and such an inhibitory effect was strengthened with the increased input dosages. Nitrate input significantly increased sediment nitrite concentration, indicating enhanced denitrification under high nitrate pressure. Bacterial network module and keystone species analysis showed that nitrate input enriched the keystone species involved in denitrification (e.g., Simplicispira and Denitratisoma). In contrast, some potential erythromycin-degrading bacteria (e.g., Desulfatiglandales, Pseudomonadales, Nitrospira) were inhibited by nitrate input. The variations in dominant bacterial groups implied competition between denitrification and erythromycin degradation in response to nitrate input. Based on the partial least squares path modeling analysis, keystone species (total effect: 0.419) and bacterial module (total effect: 0.403) showed strong association with erythromycin removal percentage. This indicated that the inhibitory effect of nitrate input on erythromycin degradation was mainly explained by bacterial network modules and keystone species. These findings will help us to assess the bioremediation potential of antibiotic-contaminated sediments suffering from excessive nitrogen discharge concurrently.

A novel incomplete hesitant fuzzy information supplement and clustering method for large-scale group decision-making.

Clustering is an effective means to reduce the scaling of large-scale group decision-making (LSGDM). However, there are many problems with clustering methods, such as incomplete or ambiguous information usually provided by different decision makers. Traditional clustering methods may not be able to handle these situations effectively, resulting in incomplete decision-making information. Calculating the clustering centers may become very complex and time-consuming. Inappropriate distance weights may also lead to incorrect cluster assignments, and these problems will seriously affect the clustering results. This research provides a novel incomplete hesitant fuzzy information supplement and clustering approach for large-scale group decision-making in order to address the aforementioned difficulties. First, the approach takes into account the trust degradation and the inhibition of relationships of distrust in the process of trust propagation, and then it builds a global and local network of trust. A novel supplemental formula is provided that takes into account the decision-preference maker's as well as the trust-neighbor's information, allowing the decision-neighbor maker's recommendation to be realized. Therefore, an improved distance function can be proposed to calculate the weights by combining the relative standard deviation theory and selecting the selected clustering centers by using the density peaks in order to optimize the selection of clustering centers and reduce the complexity and scaling of the decision. Finally, an example is presented to demonstrate how the proposed method can be applied. The consistency index and comparison experiments are used to evaluate if the suggested approach is effective and reliable.

Triggering Dual Two-electron Pathway for H2 O2 Generation by Multiple [Bi-O]n Interlayers in Ultrathin Bi12 O17 Cl2 towards Efficient Piezo-self-Fenton Catalysis.

Piezo-self-Fenton system (PESF) has been emerging as a promising water treatment technology but suffering from unsatisfied H2 O2 production efficiency. Herein, we rationally design a Bi12 O17 Cl2 piezo-catalyst with multiple [Bi-O]n interlayers towards highly efficient H2 O2 production. The introduction of [Bi3 O4.25 ] layers initiates dual two-electron pathway for H2 O2 generation by altering the interlayer properties. It is found that the additional [Bi3 O4.25 ] layers not only enhance the polarization electric field but also serve as active sites for triggering dual pathways of two-electron O2 reduction and H2 O oxidation reaction for H2 O2 production. Therefore, the Bi12 O17 Cl2 exhibits an ultrahigh rate of H2 O2 generation (7.76 mM h-1 g-1 ) in pure water. Based on the adequate H2 O2 yield, a PESF was constructed for acetaminophen (ACE) degradation with an apparent rate constant of 0.023 min-1 . This work not only presents a potential strategy of tuning the activity of bismuth based piezo-catalysts but also provides a good example on the construction of highly efficient PESF for environmental remediation by using natural mechanical energy.

Susceptibility of Cd availability in microplastics contaminated paddy soil: Influence of ferric minerals and sulfate reduction.

The combined contamination of cadmium (Cd) and microplastics (MPs) in paddy soil always occurred, while its influence on Cd availability remained unclear. This study investigated the Cd availability in Cd-MPs co-contaminated paddy soil in consideration of both ferric minerals and sulfate reduction under flooding conditions. The presence of MPs resulted in a higher Cd releasing risk, as represented by the increase in the available Cd and decrease in Fe-Mn oxide-bound Cd contents, especially on the 7th and 14th days based on the sequential extraction results. MPs facilitated the formation of Fe-organic ligands, which accelerated the reductive dissolution of iron minerals but decreased the amounts of amorphous iron minerals due to the release of dissolved organic substances into pore water. Furthermore, MPs promoted the relative abundance of sulfate-reducing bacteria (such as Streptomyces and Desulfovibrio genera), thus increasing the contents of reductive S species, which was advantageous to the co-precipitation of Fe, S, and Cd on the surface of MPs based on our experimental and statistical results. Taken together, both iron and sulfate reduction under anaerobic conditions played a critical role in Cd mobilization in Cd-MPs co-contaminated paddy fields.

Agricultural practices and ditch size drive microbial community assembly and mediate N- and P-transformation in multistage drainage networks of paddy fields: Insights from a large-scale irrigation district in eastern China.

Agricultural drainage ditches (ADDs) are ubiquitous and regarded as active zones for biogeochemical reactions and microbe-mediated pollutant removal. However, little is known about the microbial distribution and community assembly in ADDs. Here, a typical large-scale irrigation district, including five orders of farmland drainage systems (namely field, sublateral, head, branch, and trunk ditches that could efficiently remove excess water from paddy fields to downstream water bodies), was selected to investigate the ecological processes of microbial communities and N- and P-transformation processes in multistage ditches. We found that scale effects drove distinct environmental gradients and microbial community dissimilarities and that the five ordered ditches were grouped into three clusters (field vs. sublateral vs. head, branch, and trunk ditches). Specifically, the microbial communities in the field ditches located adjacent to the paddy fields were strongly selected by agricultural fertilization and irrigation drainage, enriching salt-tolerant microbes with high nitrification and inorganic P solubilization capabilities. In comparison, the sublateral ditches showed the highest removal performance for total nitrogen (13.28-55.80%) and total phosphorus (9.06-65.07%) during the growth of rice, which was mainly attributed to the enrichment of versatile microbiota (e.g., C39, Nitrospira, and Novosphingobium) as a result of the increased stochastic processes driven by the low redox potential. Notably, the specific gene (i.e., hzsB) for anaerobic ammonium oxidation in sublateral ditches was 1-2 orders of magnitude higher than in adjacent ditches, further contributing to N loss. As field water was discharged into the large-sized head, branch, and trunk ditches, the nutrient levels decreased sharply. At the same time, deterministic processes gained more importance (∼82%), leading to the flourishing of Synechococcus and increasing the potential risk of eutrophication. Overall, the microbial communities in multistage ADDs were co-shaped by agricultural practices and ditch size, which further governed the N and P removal performance. These results provide unique insights into microbiota assembly patterns and dynamics in multistage ADDs and important ecological knowledge for controlling agricultural non-point source pollutants by managing of small-sized ditches.

Gastrointestinal Degradation and Toxicity of Disinfection Byproducts in Drinking Water Using In Vitro Models and the Roles of Gut Microbiota.

Disinfection byproducts (DBPs) in drinking water are mainly exposed to the human body after oral ingestion and degradation in the gastrointestinal tract. The role of gastrointestinal degradation in the toxic effects of DBPs still needs further investigation. In this study, the degradation of five categories of DBPs (22 DBPs) in the stomach and small intestine was investigated based on a semicontinuous steady-state gastrointestinal simulation system, and 22 DBPs can be divided into three groups based on their residual proportions. The degradation of chloroacetonitrile (CAN), dibromoacetic acid (DBAA), and tetrabromopyrrole (FBPy) was further analyzed based on the Simulator of the Human Intestinal Microbial Ecosystem inoculating the gut microbiota, and approximately 60% of CAN, 45% of DBAA, and 80% of FBPy were degraded in the stomach and small intestine, followed by the complete degradation of remaining DBPs in the colon. Meanwhile, gastrointestinal degradation can reduce oxidative stress-mediated DNA damage and apoptosis induced by DBPs in DLD-1 cells, but the toxicity of DBPs did not disappear with the complete degradation of DBPs, possibly because of their interferences on gut microbiota. This study provides new insights into investigating the gastrointestinal toxic effects and mechanisms of DBPs through oral exposure.

Regulatory mechanisms of submerged macrophyte on bacterial community recovery in decabromodiphenyl ether contaminated sediment: Microbiological and metabolomic perspectives.

Polybrominated diphenyl ether contamination in sediments poses serious threats to human health and ecological safety. Despite the broad application of submerged macrophytes for remediating pollutants, their regulatory influence on bacterial communities in contaminated sediments remains unclear. Herein, we analyzed the effects of decabromodiphenyl ether (BDE-209) and Hydrilla verticillata on sediment bacterial community and function using 16S rRNA gene sequencing and sediment metabolomics. Results showed that BDE-209 significantly inhibited sediment bacterial diversity and metabolic functions. It also enhanced bacterial interactions and altered both the bacterial community and metabolite composition. Uridine and inosine were critical metabolites that positively co-occurred with bacterial taxa inhibited by BDE-209. Notably, planting H. verticillata effectively alleviated the adverse impacts of BDE-209 by reducing its residuals, increasing the total organic carbon, and modifying metabolic profiles. Such mitigation was evidenced by enhancing bacterial diversity, restoring metabolic functions, and attenuating bacterial interactions. However, mitigation effectiveness depended on treatment time. Additionally, propionic acid, palmitic acid, and palmitoleic acid may facilitate the restoration of phylum Proteobacteria and class Planctomycetacia in H. verticillata planted sediment. Together, these findings improve understanding of BDE-209's impacts on aquatic ecosystems and provide valuable insights for ecological restoration using submerged macrophytes.

Environmental energy enhanced solar-driven evaporator with spontaneous internal convection for highly efficient water purification.

Solar-driven interfacial evaporation for water purification is limited by the structural design of the solar evaporator and, more importantly, by the inability to separate the water from volatile organic compounds (VOCs) present in the water source. Here, we report a three-dimensional (3D) bifunctional evaporator based on N-doped carbon (CoNC/CF), which enables the separation of fresh water from VOCs by activating PMS during the evaporation process with a VOC removal rate of 99%. There is abundant van der Waals interaction between peroxymonosulfate (PMS) and CoNC/CF, and pyrrolic N is confirmed as the active site for binding phenol, thus contributing to the separation of phenol from water. With the advantageous features of sufficient light absorption, adequate water storage capacity, and spontaneous internal convection flow on its top surface, the 3D evaporator achieves a high evaporation rate under one sun (1 kW/m2) at 3.16 kg/m2/h. More notably, through careful structural design, additional energy from the environment and water can be utilized. With such a high evaporation rate and satisfactory purification performance, this work is expected to provide a promising platform for wastewater treatment.

Gross yield driving the mass fluxes of fishery drugs: Evidence of occurrence from full aquaculture cycle in lower Yangtze River Basin.

Expanding aquaculture has generated pollutants like fishery drugs in wastewater, which affects the aquatic environments and hinders sustainable development of aquaculture. To evaluate the occurrence, mass fluxes and production factors of fishery drugs in aquaculture, full-aquaculture-cycle monitoring in finfish and crustacean wastewater was conducted in the lower Yangtze River Basin, and 28 pesticides and 15 antibiotics were detected. The results showed that individual fishery drugs varied from ppt to ppb levels. Among them, sulfonamides were dominant with a mean concentration of 105.95 ± 4.13 ng·L-1 in finfish aquacultural wastewater, and insecticides were prevailing in crustacean aquacultural wastewater with a content of 146.56 ± 0.66 ng·L-1. Since the susceptibility to finfish disease determined the aquaculture practice, there were significant differences between two types of aquacultural wastewater. Finfish aquacultural wastewater contained more drugs and reached peak earlier in rapid-growth period, yet crustacean aquacultural wastewater peaked at the harvest period, to prevent against disease. Meanwhile, higher ecological risk, especially for florfenicol, were found in finfish wastewater. With 6 production factors from Good Aquaculture Practice, the gross yield was the most influential factor of drug mass flux, explaining 98 % variance by stepwise regression. Apart from increasing concentrations of fishery drugs in wastewater, regional high-yield aquaculture also significantly impacted the corresponding mass flux. As estimated by linear regression, 1.63 tons of target drugs would be discharged by 1 Mt. aquatic products, and 7.77 tons were discharged from aquaculture in the lower Yangtze River Basin in 2021. This is the first report to quantify mass fluxes of fishery drugs and to highlight gross yield as the most influential factor, which provides guidance for the supervision and regulation of sustainable aquaculture.

Cascade damming impacts on microbial mediated nitrogen cycling in rivers.

Rivers display essential role in nitrogen (N) cycling in terrestrial and aquatic ecosystems, but now they are suffering from damming worldwide, especially from cascade damming. Despite of the importance of microorganisms in biogeochemical nutrient cycling, little attention has been paid to microbial functional biogeography under damming disturbances. Here, the Geochip microarray was applied to investigate the microbial mediated N cycling across the single-dammed Yarlung Tsangpo-Brahmaputra River and the cascade-dammed Lancang-Mekong River in southwest China. Our results showed that the N cycling processes (nitrogen fixation, ammonification, denitrification, nitrification and anammox) were stimulated in reservoirs in both rivers and the enhancement was inversely coupled with hydraulic retention time, but the recovery of N-cycling gene abundance in downstream of dam was intervened by cascade damming. Moreover, N-cycling gene composition was significantly altered in the single-dammed river, while no remarkable change was found in the cascade-dammed reaches. However, different from the unvaried gene composition, cascade damming intervened the recovery of N-cycling gene flow connectivity and resulted in the continuous decrease of connectivity in cascade damming reaches. In addition, in the single-dammed river, nutrients were the important drivers for variation in gene abundance, while they did not influence gene composition. Meanwhile, the abundance and composition of N-cycling genes in the cascade-dammed river were both significantly correlated to geographical parameters and water physical characteristics. Therefore, our study has vital implications for anticipating microbial functional response and biogeochemical feedback to ongoing cascade damming, contributing to the protection of river ecosystems under river regulation.

Responses of Phragmites communis and its rhizosphere bacteria to different exposure sequences of molybdenum disulfide and levofloxacin.

The effect of the molybdenum disulfide (MoS2)/levofloxacin (LVF) co-exposure was explored on Phragmites communis and rhizosphere soil bacterial communities. The sequence of MoS2/LVF exposure and the different MoS2 dosages (10 mg/kg and 100 mg/kg) contributed to different degrees of effect on the plant after 42 days of exposure. The treatment with priority addition of low dosage MoS2 significantly ameliorated P. communis growth, with root length growing up to 532.22 ± 46.29 cm compared to the sole LVF stress (200.04 ± 29.13 cm). Besides, MoS2 served as an alleviator and reduced the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) in P. communis under LVF stress, and activated bacteria in rhizosphere soil. These rhizosphere soil microbes assisted in mitigating toxic pollution in the soil and inducing plant resistance to external stress, such as bacteria genera Bacillus, Microbacterium, Flavihumibacter and altererythrobacter. Potential functional profiling of bacterial community indicated the addition of MoS2 contributed to relieve the reduction in functional genes associated with amino acid metabolism and the debilitation of gram_negative and aerobic phenotypic traits caused by LVF stress. This finding reveals the effect of different exposure sequences of MoS2 nanoparticles and antibiotic for plant-soil systems.

[Distribution Characteristics and Influencing Factors of Abundant and Rare Planktonic Microeukaryotes in Jinsha River].

Planktonic microeukaryotes are usually composed of a few abundant species and a large number of rare species, which play an important role in maintaining the health and stability of aquatic ecosystems. At present, little is known about the biogeographical distribution patterns of these two groups of microeukaryotes in large damming rivers. This study analyzed the distribution patterns of abundant and rare planktonic microeukaryotes and the dominant factors affecting their spatial distributions in the Jinsha River, one of the largest rivers in southwestern China that is strongly regulated by cascade dams. The results showed that the alpha diversity of planktonic microeukaryotes in the Jinsha River was higher in the cascade dam reach than that in the upstream natural reach, and the increase in alpha diversity of rare species was larger than that of rich species. There were significant differences in microbial community composition among different river sections, and the relative abundances of the dominant genera such as Vermamoeba shared by them were also significantly different between the two river sections. The results of distance decay analysis revealed that the geographic distribution patterns of rare and abundant taxa were jointly influenced by environmental heterogeneity and dispersal limitation, and the results of variance decomposition analysis and partial Mantel further indicated that dispersal limitation was the dominant ecological process. The results provided data support for the distribution and ecological response of microorganisms in rivers in the areas lacking data in Southwest China.

[Effects of Reservoir Water Depth on Different Plankton Communities and Keystone Species of Network Interaction].

In order to understand the impacts of the reservoir construction on the diversity and ecological network of different microbial communities, seven sampling sites were set up in the Hengshan Reservoir in 2021. Water samples were collected from the surface and bottom of the reservoir. After filtering and extracting total DNA samples, high-throughput sequencing was carried out based on 16S and 18S rDNA to investigate the response of community structure, molecular ecological network, and keystone species of different microbial groups to water environment changes. The results showed that the Richness, Simpson, Shannon, and Pielou's Evenness indices of bacterial community in the surface and bottom layers were higher than those in the eukaryote community. The dominant community of bacteria included Proteobacteria, Actinobacteria, and Bacteroidetes, and the eukaryote community included Arthropoda, Ciliophora, Ochrophyta, etc. Moreover, the density and average clustering coefficient of the microbial networks in the surface waters of different phytoplankton communities were higher than those in the bottom waters. It was also observed that the microbial ecological networks in the surface waters were more closely related, and the number of nodes and edges, as well as the number of keystone species, of bacterial communities in the surface and bottom layers were significantly higher than those in the eukaryote microbial communities, indicating that the bacterial community network was larger, and the cooperative relationship and network connectivity between species were stronger. The interaction between bacterial community and eukaryote community in different water depths was dominated by positive correlation, and the negative correlation of the two groups in the bottom layer was slightly greater than that in the surface, indicating that the competition between bottom-layer species was greater than that between surface-layer species. In addition, the environmental impact factors of all species and keystone species of the community in surface water were basically the same, but they differed greatly in deep water, indicating that the influence mechanism of water depth change on keystone species was not the same as that of all species. The results further revealed the effects of reservoir construction on the stability and interspecific interactions of different microbial communities and provided a theoretical basis for predicting variations in microbial community and material cycling in reservoirs.

Highly Efficient FeIII -initiated Self-cycled Fenton System in Piezo-catalytic Process for Organic Pollutants Degradation.

Piezo-catalytic self-Fenton (PSF) system has been emerging as a promising technique for wastewater treatment, while the competing O2 reductive hydrogen peroxide (H2 O2 ) production and FeIII reduction seriously limited the reaction kinetics. Here, we develop a two-electron water oxidative H2 O2 production (WOR-H2 O2 ) coupled with FeIII reduction over a FeIII /BiOIO3 piezo-catalyst for highly efficient PSF. It is found that the presence of FeIII can simultaneously initiate the WOR-H2 O2 and reduction of FeIII to FeII , thereby enabling a rapid reaction kinetics towards subsequent Fenton reaction of H2 O2 /FeII . The FeIII initiating PSF system offers exceptional self-recyclable degradation of pollutants with a degradation rate constant for sulfamethoxazole (SMZ) over 3.5 times as that of the classic FeII -PSF system. This study offers a new perspective for constructing efficient PSF systems and shatters the preconceived notion of FeIII in the Fenton reaction.